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htb63 human melanoma cell line  (ATCC)


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    ATCC htb63 human melanoma cell line
    ( A , B ) The panels show the gene expression of WNT5A and IL-6 in multiple microarray data sets of melanoma cell lines classified by the Heuristic Online Phenotype Prediction (HOPP) algorithm as proliferative versus invasive. ( C ) QPCR analyses of endogenous WNT5A mRNA expression levels in human WM852, <t>HTB63,</t> and A375 cells. Samples were normalised against TATA-binding protein (TBP) mRNA expression, and the results are presented relative to the WNT5A mRNA expression of WM852 cells ( n = 5). ( D ) Western blot analysis showing the levels of endogenous WNT5A protein expression in the human melanoma cell lines WM852, HTB63, and A375. α-tubulin was used as a loading control. A representative blot from four separate experiments is shown. The lower panel shows densitometric analyses of WNT5A protein expression normalised against α-tubulin; the results are presented relative to WNT5A protein expression of WM852 cells ( n = 4). ( E ) QPCR analysis of endogenous IL-6 mRNA expression levels in human WM852, HTB63, and A375 cells. Samples were normalised against TATA-binding protein (TBP) mRNA expression; the results are presented relative to the IL-6 mRNA expression of WM852 cells ( n = 5). ( F ) ELISA analysis showing the quantification of endogenous IL-6 protein secretion in human WM852, HTB63, and A375 cells ( n = 5). The results are presented as the means and S.E.Ms; *** p < 0.001.
    Htb63 Human Melanoma Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 201 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/htb63+human+melanoma+cell+line/HT-144/pmc05122349-113-2-22
    Average 95 stars, based on 201 article reviews
    htb63 human melanoma cell line - by Bioz Stars, 2026-09
    95/100 stars

    Images

    1) Product Images from "Demonstration of a WNT5A-IL-6 positive feedback loop in melanoma cells: Dual interference of this loop more effectively impairs melanoma cell invasion"

    Article Title: Demonstration of a WNT5A-IL-6 positive feedback loop in melanoma cells: Dual interference of this loop more effectively impairs melanoma cell invasion

    Journal: Oncotarget

    doi: 10.18632/oncotarget.9332

    ( A , B ) The panels show the gene expression of WNT5A and IL-6 in multiple microarray data sets of melanoma cell lines classified by the Heuristic Online Phenotype Prediction (HOPP) algorithm as proliferative versus invasive. ( C ) QPCR analyses of endogenous WNT5A mRNA expression levels in human WM852, HTB63, and A375 cells. Samples were normalised against TATA-binding protein (TBP) mRNA expression, and the results are presented relative to the WNT5A mRNA expression of WM852 cells ( n = 5). ( D ) Western blot analysis showing the levels of endogenous WNT5A protein expression in the human melanoma cell lines WM852, HTB63, and A375. α-tubulin was used as a loading control. A representative blot from four separate experiments is shown. The lower panel shows densitometric analyses of WNT5A protein expression normalised against α-tubulin; the results are presented relative to WNT5A protein expression of WM852 cells ( n = 4). ( E ) QPCR analysis of endogenous IL-6 mRNA expression levels in human WM852, HTB63, and A375 cells. Samples were normalised against TATA-binding protein (TBP) mRNA expression; the results are presented relative to the IL-6 mRNA expression of WM852 cells ( n = 5). ( F ) ELISA analysis showing the quantification of endogenous IL-6 protein secretion in human WM852, HTB63, and A375 cells ( n = 5). The results are presented as the means and S.E.Ms; *** p < 0.001.
    Figure Legend Snippet: ( A , B ) The panels show the gene expression of WNT5A and IL-6 in multiple microarray data sets of melanoma cell lines classified by the Heuristic Online Phenotype Prediction (HOPP) algorithm as proliferative versus invasive. ( C ) QPCR analyses of endogenous WNT5A mRNA expression levels in human WM852, HTB63, and A375 cells. Samples were normalised against TATA-binding protein (TBP) mRNA expression, and the results are presented relative to the WNT5A mRNA expression of WM852 cells ( n = 5). ( D ) Western blot analysis showing the levels of endogenous WNT5A protein expression in the human melanoma cell lines WM852, HTB63, and A375. α-tubulin was used as a loading control. A representative blot from four separate experiments is shown. The lower panel shows densitometric analyses of WNT5A protein expression normalised against α-tubulin; the results are presented relative to WNT5A protein expression of WM852 cells ( n = 4). ( E ) QPCR analysis of endogenous IL-6 mRNA expression levels in human WM852, HTB63, and A375 cells. Samples were normalised against TATA-binding protein (TBP) mRNA expression; the results are presented relative to the IL-6 mRNA expression of WM852 cells ( n = 5). ( F ) ELISA analysis showing the quantification of endogenous IL-6 protein secretion in human WM852, HTB63, and A375 cells ( n = 5). The results are presented as the means and S.E.Ms; *** p < 0.001.

    Techniques Used: Gene Expression, Microarray, Expressing, Binding Assay, Western Blot, Control, Enzyme-linked Immunosorbent Assay

    Matrigel invasion of WM852 ( A and B ) and HTB63 ( C and D ) cells treated with either Box5 or neutralising anti-IL-6 antibody alone and their combination was evaluated as described under the Materials and Methods section. Proper controls (DMSO for Box5 treatment or IgG 1 isotype antibody for neutralising anti-IL-6-antibody treatment) were used to compare the treatment effect on the cell invasions. The invasion assays ( n = 5) were performed over 24 h and analysed as described in the Materials and Methods section. The results are presented as the means and S.E.Ms; * p < 0.05; ** p < 0.01; *** p < 0.001.
    Figure Legend Snippet: Matrigel invasion of WM852 ( A and B ) and HTB63 ( C and D ) cells treated with either Box5 or neutralising anti-IL-6 antibody alone and their combination was evaluated as described under the Materials and Methods section. Proper controls (DMSO for Box5 treatment or IgG 1 isotype antibody for neutralising anti-IL-6-antibody treatment) were used to compare the treatment effect on the cell invasions. The invasion assays ( n = 5) were performed over 24 h and analysed as described in the Materials and Methods section. The results are presented as the means and S.E.Ms; * p < 0.05; ** p < 0.01; *** p < 0.001.

    Techniques Used:

    Related Articles

    Gene Expression:

    Article Title: Demonstration of a WNT5A-IL-6 positive feedback loop in melanoma cells: Dual interference of this loop more effectively impairs melanoma cell invasion
    Article Snippet: The A375 human melanoma cell line originates from a metastatic site and its genetic background includes BRAF V600E /NRAS WT /PTEN WT /TP53 WT .The A375 human melanoma cell line originates from a metastatic site and its genetic background includes BRAF V600E /NRAS WT /PTEN WT /TP53 WT .. Both the HTB63 human melanoma cell line (Cat#HT-144, LOT: 59550354) and the A375 human melanoma cell line (Cat#CRL-1619, LOT:61573377) were obtained from American Type Culture Collection (ATCC, VA, USA) in August 2014.. The identities of the HTB63 and A375 cell lines were confirmed by ATCC by using multiplex PCR, which amplifies the amelogenin gene and eight of the most informative polymorphic markers in the human genome.The identities of the HTB63 and A375 cell lines were confirmed by ATCC by using multiplex PCR, which amplifies the amelogenin gene and eight of the most informative polymorphic markers in the human genome.

    Microarray:

    Article Title: Demonstration of a WNT5A-IL-6 positive feedback loop in melanoma cells: Dual interference of this loop more effectively impairs melanoma cell invasion
    Article Snippet: The A375 human melanoma cell line originates from a metastatic site and its genetic background includes BRAF V600E /NRAS WT /PTEN WT /TP53 WT .The A375 human melanoma cell line originates from a metastatic site and its genetic background includes BRAF V600E /NRAS WT /PTEN WT /TP53 WT .. Both the HTB63 human melanoma cell line (Cat#HT-144, LOT: 59550354) and the A375 human melanoma cell line (Cat#CRL-1619, LOT:61573377) were obtained from American Type Culture Collection (ATCC, VA, USA) in August 2014.. The identities of the HTB63 and A375 cell lines were confirmed by ATCC by using multiplex PCR, which amplifies the amelogenin gene and eight of the most informative polymorphic markers in the human genome.The identities of the HTB63 and A375 cell lines were confirmed by ATCC by using multiplex PCR, which amplifies the amelogenin gene and eight of the most informative polymorphic markers in the human genome.

    Expressing:

    Article Title: Demonstration of a WNT5A-IL-6 positive feedback loop in melanoma cells: Dual interference of this loop more effectively impairs melanoma cell invasion
    Article Snippet: The A375 human melanoma cell line originates from a metastatic site and its genetic background includes BRAF V600E /NRAS WT /PTEN WT /TP53 WT .The A375 human melanoma cell line originates from a metastatic site and its genetic background includes BRAF V600E /NRAS WT /PTEN WT /TP53 WT .. Both the HTB63 human melanoma cell line (Cat#HT-144, LOT: 59550354) and the A375 human melanoma cell line (Cat#CRL-1619, LOT:61573377) were obtained from American Type Culture Collection (ATCC, VA, USA) in August 2014.. The identities of the HTB63 and A375 cell lines were confirmed by ATCC by using multiplex PCR, which amplifies the amelogenin gene and eight of the most informative polymorphic markers in the human genome.The identities of the HTB63 and A375 cell lines were confirmed by ATCC by using multiplex PCR, which amplifies the amelogenin gene and eight of the most informative polymorphic markers in the human genome.

    Binding Assay:

    Article Title: Demonstration of a WNT5A-IL-6 positive feedback loop in melanoma cells: Dual interference of this loop more effectively impairs melanoma cell invasion
    Article Snippet: The A375 human melanoma cell line originates from a metastatic site and its genetic background includes BRAF V600E /NRAS WT /PTEN WT /TP53 WT .The A375 human melanoma cell line originates from a metastatic site and its genetic background includes BRAF V600E /NRAS WT /PTEN WT /TP53 WT .. Both the HTB63 human melanoma cell line (Cat#HT-144, LOT: 59550354) and the A375 human melanoma cell line (Cat#CRL-1619, LOT:61573377) were obtained from American Type Culture Collection (ATCC, VA, USA) in August 2014.. The identities of the HTB63 and A375 cell lines were confirmed by ATCC by using multiplex PCR, which amplifies the amelogenin gene and eight of the most informative polymorphic markers in the human genome.The identities of the HTB63 and A375 cell lines were confirmed by ATCC by using multiplex PCR, which amplifies the amelogenin gene and eight of the most informative polymorphic markers in the human genome.

    Western Blot:

    Article Title: Demonstration of a WNT5A-IL-6 positive feedback loop in melanoma cells: Dual interference of this loop more effectively impairs melanoma cell invasion
    Article Snippet: The A375 human melanoma cell line originates from a metastatic site and its genetic background includes BRAF V600E /NRAS WT /PTEN WT /TP53 WT .The A375 human melanoma cell line originates from a metastatic site and its genetic background includes BRAF V600E /NRAS WT /PTEN WT /TP53 WT .. Both the HTB63 human melanoma cell line (Cat#HT-144, LOT: 59550354) and the A375 human melanoma cell line (Cat#CRL-1619, LOT:61573377) were obtained from American Type Culture Collection (ATCC, VA, USA) in August 2014.. The identities of the HTB63 and A375 cell lines were confirmed by ATCC by using multiplex PCR, which amplifies the amelogenin gene and eight of the most informative polymorphic markers in the human genome.The identities of the HTB63 and A375 cell lines were confirmed by ATCC by using multiplex PCR, which amplifies the amelogenin gene and eight of the most informative polymorphic markers in the human genome.

    Control:

    Article Title: Demonstration of a WNT5A-IL-6 positive feedback loop in melanoma cells: Dual interference of this loop more effectively impairs melanoma cell invasion
    Article Snippet: The A375 human melanoma cell line originates from a metastatic site and its genetic background includes BRAF V600E /NRAS WT /PTEN WT /TP53 WT .The A375 human melanoma cell line originates from a metastatic site and its genetic background includes BRAF V600E /NRAS WT /PTEN WT /TP53 WT .. Both the HTB63 human melanoma cell line (Cat#HT-144, LOT: 59550354) and the A375 human melanoma cell line (Cat#CRL-1619, LOT:61573377) were obtained from American Type Culture Collection (ATCC, VA, USA) in August 2014.. The identities of the HTB63 and A375 cell lines were confirmed by ATCC by using multiplex PCR, which amplifies the amelogenin gene and eight of the most informative polymorphic markers in the human genome.The identities of the HTB63 and A375 cell lines were confirmed by ATCC by using multiplex PCR, which amplifies the amelogenin gene and eight of the most informative polymorphic markers in the human genome.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Demonstration of a WNT5A-IL-6 positive feedback loop in melanoma cells: Dual interference of this loop more effectively impairs melanoma cell invasion
    Article Snippet: The A375 human melanoma cell line originates from a metastatic site and its genetic background includes BRAF V600E /NRAS WT /PTEN WT /TP53 WT .The A375 human melanoma cell line originates from a metastatic site and its genetic background includes BRAF V600E /NRAS WT /PTEN WT /TP53 WT .. Both the HTB63 human melanoma cell line (Cat#HT-144, LOT: 59550354) and the A375 human melanoma cell line (Cat#CRL-1619, LOT:61573377) were obtained from American Type Culture Collection (ATCC, VA, USA) in August 2014.. The identities of the HTB63 and A375 cell lines were confirmed by ATCC by using multiplex PCR, which amplifies the amelogenin gene and eight of the most informative polymorphic markers in the human genome.The identities of the HTB63 and A375 cell lines were confirmed by ATCC by using multiplex PCR, which amplifies the amelogenin gene and eight of the most informative polymorphic markers in the human genome.



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    htb63 human melanoma cell line  (ATCC)
    95
    ATCC htb63 human melanoma cell line
    ( A , B ) The panels show the gene expression of WNT5A and IL-6 in multiple microarray data sets of melanoma cell lines classified by the Heuristic Online Phenotype Prediction (HOPP) algorithm as proliferative versus invasive. ( C ) QPCR analyses of endogenous WNT5A mRNA expression levels in human WM852, <t>HTB63,</t> and A375 cells. Samples were normalised against TATA-binding protein (TBP) mRNA expression, and the results are presented relative to the WNT5A mRNA expression of WM852 cells ( n = 5). ( D ) Western blot analysis showing the levels of endogenous WNT5A protein expression in the human melanoma cell lines WM852, HTB63, and A375. α-tubulin was used as a loading control. A representative blot from four separate experiments is shown. The lower panel shows densitometric analyses of WNT5A protein expression normalised against α-tubulin; the results are presented relative to WNT5A protein expression of WM852 cells ( n = 4). ( E ) QPCR analysis of endogenous IL-6 mRNA expression levels in human WM852, HTB63, and A375 cells. Samples were normalised against TATA-binding protein (TBP) mRNA expression; the results are presented relative to the IL-6 mRNA expression of WM852 cells ( n = 5). ( F ) ELISA analysis showing the quantification of endogenous IL-6 protein secretion in human WM852, HTB63, and A375 cells ( n = 5). The results are presented as the means and S.E.Ms; *** p < 0.001.
    Htb63 Human Melanoma Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/htb63+human+melanoma+cell+line/HT-144/pmc05122349-113-2-22
    Average 95 stars, based on 1 article reviews
    htb63 human melanoma cell line - by Bioz Stars, 2026-09
    95/100 stars
    		  Buy from Supplier

    Image Search Results


    ( A , B ) The panels show the gene expression of WNT5A and IL-6 in multiple microarray data sets of melanoma cell lines classified by the Heuristic Online Phenotype Prediction (HOPP) algorithm as proliferative versus invasive. ( C ) QPCR analyses of endogenous WNT5A mRNA expression levels in human WM852, HTB63, and A375 cells. Samples were normalised against TATA-binding protein (TBP) mRNA expression, and the results are presented relative to the WNT5A mRNA expression of WM852 cells ( n = 5). ( D ) Western blot analysis showing the levels of endogenous WNT5A protein expression in the human melanoma cell lines WM852, HTB63, and A375. α-tubulin was used as a loading control. A representative blot from four separate experiments is shown. The lower panel shows densitometric analyses of WNT5A protein expression normalised against α-tubulin; the results are presented relative to WNT5A protein expression of WM852 cells ( n = 4). ( E ) QPCR analysis of endogenous IL-6 mRNA expression levels in human WM852, HTB63, and A375 cells. Samples were normalised against TATA-binding protein (TBP) mRNA expression; the results are presented relative to the IL-6 mRNA expression of WM852 cells ( n = 5). ( F ) ELISA analysis showing the quantification of endogenous IL-6 protein secretion in human WM852, HTB63, and A375 cells ( n = 5). The results are presented as the means and S.E.Ms; *** p < 0.001.

    Journal: Oncotarget

    Article Title: Demonstration of a WNT5A-IL-6 positive feedback loop in melanoma cells: Dual interference of this loop more effectively impairs melanoma cell invasion

    doi: 10.18632/oncotarget.9332

    Figure Lengend Snippet: ( A , B ) The panels show the gene expression of WNT5A and IL-6 in multiple microarray data sets of melanoma cell lines classified by the Heuristic Online Phenotype Prediction (HOPP) algorithm as proliferative versus invasive. ( C ) QPCR analyses of endogenous WNT5A mRNA expression levels in human WM852, HTB63, and A375 cells. Samples were normalised against TATA-binding protein (TBP) mRNA expression, and the results are presented relative to the WNT5A mRNA expression of WM852 cells ( n = 5). ( D ) Western blot analysis showing the levels of endogenous WNT5A protein expression in the human melanoma cell lines WM852, HTB63, and A375. α-tubulin was used as a loading control. A representative blot from four separate experiments is shown. The lower panel shows densitometric analyses of WNT5A protein expression normalised against α-tubulin; the results are presented relative to WNT5A protein expression of WM852 cells ( n = 4). ( E ) QPCR analysis of endogenous IL-6 mRNA expression levels in human WM852, HTB63, and A375 cells. Samples were normalised against TATA-binding protein (TBP) mRNA expression; the results are presented relative to the IL-6 mRNA expression of WM852 cells ( n = 5). ( F ) ELISA analysis showing the quantification of endogenous IL-6 protein secretion in human WM852, HTB63, and A375 cells ( n = 5). The results are presented as the means and S.E.Ms; *** p < 0.001.

    Article Snippet: Both the HTB63 human melanoma cell line (Cat#HT-144, LOT: 59550354) and the A375 human melanoma cell line (Cat#CRL-1619, LOT:61573377) were obtained from American Type Culture Collection (ATCC, VA, USA) in August 2014.

    Techniques: Gene Expression, Microarray, Expressing, Binding Assay, Western Blot, Control, Enzyme-linked Immunosorbent Assay

    Matrigel invasion of WM852 ( A and B ) and HTB63 ( C and D ) cells treated with either Box5 or neutralising anti-IL-6 antibody alone and their combination was evaluated as described under the Materials and Methods section. Proper controls (DMSO for Box5 treatment or IgG 1 isotype antibody for neutralising anti-IL-6-antibody treatment) were used to compare the treatment effect on the cell invasions. The invasion assays ( n = 5) were performed over 24 h and analysed as described in the Materials and Methods section. The results are presented as the means and S.E.Ms; * p < 0.05; ** p < 0.01; *** p < 0.001.

    Journal: Oncotarget

    Article Title: Demonstration of a WNT5A-IL-6 positive feedback loop in melanoma cells: Dual interference of this loop more effectively impairs melanoma cell invasion

    doi: 10.18632/oncotarget.9332

    Figure Lengend Snippet: Matrigel invasion of WM852 ( A and B ) and HTB63 ( C and D ) cells treated with either Box5 or neutralising anti-IL-6 antibody alone and their combination was evaluated as described under the Materials and Methods section. Proper controls (DMSO for Box5 treatment or IgG 1 isotype antibody for neutralising anti-IL-6-antibody treatment) were used to compare the treatment effect on the cell invasions. The invasion assays ( n = 5) were performed over 24 h and analysed as described in the Materials and Methods section. The results are presented as the means and S.E.Ms; * p < 0.05; ** p < 0.01; *** p < 0.001.

    Article Snippet: Both the HTB63 human melanoma cell line (Cat#HT-144, LOT: 59550354) and the A375 human melanoma cell line (Cat#CRL-1619, LOT:61573377) were obtained from American Type Culture Collection (ATCC, VA, USA) in August 2014.

    Techniques: